Abstract

Glyceric acid (GA) is a human metabolite existing in L- and D-configurations, which are considered the markers for the diseases L- and D-glyceric aciduria/academia, respectively. Enantioselective, potentiometric membrane electrodes based on carbon paste modified with antibiotics as chiral selectors, vancomycin, and teicoplanin were designed for the assay of L- and D-GA, respectively, in the concentration ranges of 10−9–10−7and 10−4–10−2 moL/L with very low detection limits (1.5 × 10−10 moL/L for L-GA and 1.6 × 10−4 moL/L for D-GA, resp.). The surface of the electrodes can be regenerated simply by polishing in order to obtain a fresh surface ready to be used in a new assay. The proposed electrodes can be successfully applied for the enantioanalysis of L- and D-glyceric acids in serum samples.

Highlights

  • The enantiomers of the urinary organic acids are important markers for inborn errors of metabolism

  • This paper describes the design, response characteristics, selectivity, and applications of two EPMEs based on vancomycin and teicoplanin for the enantioanalysis of Glyceric acid (GA)

  • The response obtained for L-GA was linear and near-Nernstian only for the EPME based on vancomycin, while the response obtained for D-GA was linear and near-Nernstian only for the EPME based on teicoplanin

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Summary

Introduction

The enantiomers of the urinary organic acids are important markers for inborn errors of metabolism. Glyceric acid (2,3-dihydroxypropionic acid, GA) is a human metabolite existing in L- and D-configurations. These two enantiomers are vital biological markers for the diagnosis of two different metabolic diseases, primary hyperoxaluria type II (L-glyceric aciduria, PH2) and D-glyceric aciduria [1,2,3,4,5,6]. Enantioselective analysis of glyceric acid is necessary to differentiate between the two inherited metabolic diseases. Macrocyclic antibiotics represent a new class of chiral selectors used in the design of EPME, offering a high selectivity and enantioselectivity [17]. 206.0 a All measurements were made at 25◦C; all values are the average of ten determinations

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