Enantiomeric Separation of a Series of β‐Lactams Using Capillary Zone Electrophoresis

Abstract

Twelve racemic substituted β‐lactam compounds were examined via capillary zone electrophoresis using three chiral selectors: sulfated α‐cyclodextrin (SAC), sulfated β‐cyclodextrin (SBC), and carboxymethyl β‐cyclodextrin (CMBC). Ten of the twelve β‐lactams are separated and each of the ten compounds is baseline separated by at least one of the chiral run buffer additives under optimized conditions. SAC was found to be the most effective chiral selector, baseline separating seven of the analytes and partially separating another two. The concentration of the chiral selector had a prominent effect on the resolution, generally higher concentrations gave longer migration times and better resolutions. Addition of organic modifier also increased analyses time but gave lower resolution. Decreasing the pH of the run buffer generally decreased analyses times as well as resolution. Decreasing the applied voltage generally improved resolution.