EN

Abstract

Present study was conducted to evaluate the effect of melatonin (MEL) on velocity and motility of mithun semen by computer assisted sperm analyser. Semen ejaculates (n=25) were collected from matured mithun bulls (n=10) using trans-rectal massage method and were divided and grouped into six aliquots equally, extended with the standard Tris Egg Yolk Citrate semen diluent. Six groups for various treatments were prepare, these group were control - semen without MEL (Gr 1), 1mM (Gr 2), 2mM (Gr 3), 3Mm (Gr 4), 4mM (Gr 5:) and 5mM (Gr 6). Various parameter such as total motility, forward progressive motility, straight line velocity, curvilinear velocity, average path velocity, wobble, linearity, straightness, beat/cross frequency, amplitude of lateral head displacement and velocity of rapid, medium, slow and static were measured for 0-30h at 6 hrs interval at 5 o C. The result observed that these mobility and velocity parameters were varied significantly (p<0.05) among the experimental periods and among the experimental groups. Further, MEL at 3 mM has significant (p<0.05) improvement in the mobility and velocity parameters than MEL at 1, 2, 4or 5 mM stored in in- vitro for up to 30 h of incubation. It was concluded that MEL 3 mM treated sperm has increased functional sperm structures faster to move and forward direction, probably improves the fertilization rate.