Abstract
The present study was undertaken to assess the motility and velocity parameters of sperm of freezable and nonfreezable ejaculates by computer-assisted sperm analyser (CASA) such as Hamilton-Thorne Semen Analyser IVOS 11 in mithun semen. Fifty ejaculates (twenty-five ejaculates each for freezable and nonfreezable semen ejaculates) were collected from ten matured mithun bulls. CASA parameters, motility parameters such as forward progressive motility (FPM) (%), nonprogressive motility (NPM) (%), total motility (TM) (%), and static sperms (SM) (%); velocity parameters such as curvilinear velocity (VCL) (μm/sec), straight line velocity (VSL) (μm/sec), average path velocity (VAP) (μm/sec), linearity (LIN) (%), straightness (STR) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (μm), and beat/cross-frequency (BCF) (Hz) were measured by CASA analyser. The result revealed that these parameters varied significantly (P<0.05) between the freezable and nonfreezable ejaculates and freezable ejaculates have significantly (P<0.05) higher value than nonfreezable ejaculates. It was concluded that most of the CASA parameters were significantly lower in nonfreezable ejaculates than in freezable ejaculates in mithun and confirmed that the CASA was effective for a quick and objective analysis of motility and velocity parameters in mithun semen.
Highlights
Mithun (Bos frontalis) is a semiwild, free-range, rare bovine species present in the North-Eastern Hill (NEH) region of India and is believed to have originated more than 8000 years ago from wild Indian gaur (Bos gaurus) [1]
The objective of this study was to assess the motility and velocity parameters measured by computer-assisted sperm analyser (CASA) to pursuit future sperm preservation protocols in mithun
The percent of total motility (TM) and forward progressive motility (FPM) were significantly (P < 0.05) higher in freezable ejaculates than in nonfreezable ejaculates and nonprogressive motility (NPM) and SM were significantly higher in nonfreezable ejaculates than in freezable ejaculates
Summary
Mithun (Bos frontalis) is a semiwild, free-range, rare bovine species present in the North-Eastern Hill (NEH) region of India and is believed to have originated more than 8000 years ago from wild Indian gaur (Bos gaurus) [1]. The animal has an important place in the social, cultural, religious, and economic life of the tribal population of NEH region. As per the livestock census of India (2007), the mithun population is decreasing gradually due to lack of suitable breeding bulls, increase in intensive inbreeding practices, and lack of suitable breeding management. Greater efforts are required from all quarters to preserve the mithun population to enhance the socioeconomic status of this region. Since mithuns are semiwild animal and not fully domesticated, natural breeding is practiced in this species with accompanied limitations like cost and disease transmission. Use of artificial insemination for improvement of its pedigree is utmost essential
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