Abstract
ABSTRACTThe possibility for induction of in vitro embryogenesis on solid culture medium from seedless grape cultivars and hybrid combinations, giving an account of the effect of explant age, genotype and growth regulators was investigated. We established that in all studied treatments and genotypes explants isolated 3 days after pollination formed only callus. Comparing the capability for embryo induction, seed buds isolated 10 and 30 days after pollination showed increased regeneration capacity with specifics toward development of somatic or zygotic embryo. The frequency of zygotic embryo formation was higher in the inbreed treatments while the hybrid genotypes produced more somatic embryos. Converting into green plants suitable for micropropagation was observed only from explants isolated 10 and 30 days after pollination. The best embryogenic ability was established for Rusalka3-inbred and the hybrid combination Russalka X Alicante Buchet. Regarding the effect of the culture media the higher concentration of growth regulators in ICI did not promote better frequency of embryo formation than I3 medium, but resulted in obtaining of more green plants with normal development and growth.
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