Abstract
ABSTRACTIt was investigated the propensity for induction of somatic embryogenesis in vitro from seedless grape cultivars and hybrid combinations in liquid culture giving an account of the effect of explant age, genotype and growth regulators in the culture medium. We established that embryogenic structures were formed in all studied treatments and genotypes but there were specifics toward development of somatic or zygotic embryo. Comparing the capability for embryo formation, seed buds isolated 10 and 30 days after pollination showed increased regeneration capacity. Following the experimental protocol the best embryogenic ability was established for Rusalka3-inbred and the hybrid combination Russalka X Alicante Buchet. Regarding the effect of the growth regulators NOA in combination with BA promoted better frequency of embryo formation than 2,4-D. The genotype Russalka 3 demonstrated highest propensity for embryogenesis in vitro.
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