Abstract
When cultured on agar-solidified media (based on Murashige and Skoog's formula), immature inflorescences and nodes ofLolium multiflorum underwent several different pathways of morphogenesis. The pathway expressed was dependent upon the type of explant, its age and the composition of the culture medium. Immature inflorescences generally produced either leaves and roots or embryoids whereas nodes produced axillary shoots or embryoids. Embryogenesis from both explant sources occurred from a firm, white, opaque, proliferating tissue. The embryoids could be cultured individually and induced to produce plantlets capable of transfer to soil. Generally the plantlets formed from newly isolated tissues were green, but chlorophyll-deficient plantlets arose infrequently (up to about 5%). However, this frequency increased as the embryoid-producing tissues were propagated by subculture.
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