Abstract
Twenty-two superovulated IBRV-seronegative donors were infected intranasally, intravaginally or per uterus with IBRV prior to being inseminated with semen from seronegative bulls. All of the donors became infected and shed IBRV. Sixty-three embryos, collected non-surgically 7 and 8 days after estrus, were washed, treated with trypsin for 1 minute and transferred to 49 synchronized IBRV-seronegative recipients. Twenty-one pregnancies resulted. Three months later, 11 of the 22 donors were treated with dexamethasone to induce virus shedding. This drug inhibited ovulation, however, and only one embryo, which resulted in a pregnancy when transferred, was collected. Of the 22 pregnancies, one was accidently aborted, a second spontaneously aborted, one calf and two sets of twins were stillborn and 20 live calves were delivered. All recipients and calves remained serologically negative for antibodies to IBRV. Virus isolation and histopathology on both abortuses, the stillborn calves, and three calves slaughtered at three months of age were negative for IBRV.
Published Version
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