Elutriation of Blood Cell Components and Mast Cells by Nonsynchronous Coil Planet Centrifuge

Abstract

The nonsynchronous coil planet centrifuge was applied to the separation of blood cell components and mast cells using a physiological buffer solution. The optimal separation of sheep blood samples was performed at the counterclockwise revolution and the clockwise rotation of coiled separation column by the head to tail elution mode. Under the high speed of revolution of 600–1000 rpm, sheep and human blood samples were completely separated and eluted into two peaks, where all cell components, other than erythrocytes such as platelets and leukocytes, were eluted at 0 rpm and erythrocytes at 10 rpm of coil rotation. The normal elution pattern was achieved at 800 rpm of revolution for sheep blood and at 700 rpm for human blood samples. The separation of mast cells, large cells with a high density, was also performed using an eluent composed of RPMI 1640 medium+10% FCS. Mast cells were satisfactorily separated from other cell components under a high speed revolution combined with a low speed of coil rotation (2–5 rpm). The overall results indicate that the nonsynchronous CPC can be effectively used for separation of blood cell components and mast cells according to their sedimentation rates.