Abstract
Abstract A simplified method of preparing tobacco samples for calcium determination was sought. Timed leachings of tobacco indicated that cation exchange phenomena operated in systems containing calcium, potassium, tobacco cellulose, and water. Affinities of the cations involved were such that hydrogen ion exchanged with calcium and displaced it into solution. An elutive treatment was developed, based on this property, whereby the readsorption of calcium could be successfully reduced. A radiation buffer technique of flame photometric examination eliminated the effect of potassium interference on calcium determination, allowing calcium and potassium to be run on the same eluate when treated as follows: Tobacco is mixed with acid-washed Celite 545, topped with pure acid-washed Celite 545, and eluted with 3N HCl. The eluate is made to 1000 ml and a portion is withdrawn and compared against standards containing the equivalent amount of acid for potassium determination. A 50 ml aliquot of eluate is then made to 100 ml with sufficient potassium of any convenient concentration, such as 15 ml of 2000 ppm potassium, so as to yield a final solution containing 300 ppm K. Calcium is compared to similarly prepared flame photometry standards.
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