Abstract
Agrobacterium tumefaciens F/1 produces two different O-chains, both are constituted of rhamnose and glucosamine: the less abundant has a linear disaccharidic repeating unit 3)-α- l-Rha p-(1 → 3)-β- d-Glc pNAc-(1→ and the second one 4)-α- l-Rha p-(1 → 3)-β- d-Glc pNAc-(1→. The two intact antigenic moieties were studied in mixture by 2D NMR. Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected.
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