Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells.

Thouria Bourhim,Hiroko Isoda,Myra O Villareal,Chemseddoha Gadhi

doi:10.3390/nu13082697

Thouria Bourhim, Hiroko Isoda + Show 2 more

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https://doi.org/10.3390/nu13082697

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Journal: Nutrients	Publication Date: Aug 4, 2021
Citations: 11	License type: CC BY 4.0

Affiliation: Cadi Ayyad University, University of Tsukuba

Abstract

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

Highlights

Argania spinosa L. is a Moroccan endemic tree
The processes down-regulated by argan presspress-cake (APC) extract included those that are associated with pigmentation, melanosome transport, keratinocyte differentiation, melanocyte differentiation, cell differentiation, and apoptosis
Nervous system development, Wnt receptor signaling pathway through beta-catenin, the glutathione metabolic process, and MAP kinase kinase kinase activity were differentially regulated by APC extract

Summary

Introduction

Argania spinosa L. (family Sapotaceae) is a Moroccan endemic tree. It covers more than800,000 hectares in the south-western region of the country [1]. (family Sapotaceae) is a Moroccan endemic tree. 800,000 hectares in the south-western region of the country [1]. Argan oil is extremely rich in unsaturated fatty acids and bioactive phytochemicals including tocopherols, phenolic compounds, and carotenoids [3]. Contributing to its pharmacological and cosmetic properties [4]. This oil is used as an anti-aging skin care [5]. Consumption of argan oil and/or topical application decrease trans-epidermal water loss and improve skin elasticity [6,7]

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