Elucidation of Antimicrobial Silver Sulfadiazine (SSD) Blend/Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) Immobilised with Collagen Peptide as Potential Biomaterial.

Sevakumaran Vigneswari,Seeram Ramakrishna,Al-Ashraf Abdullah Amirul,Tana Poorani Gurusamy,H P S Abdul Khalil

doi:10.3390/polym12122979

Abstract

The quest for a suitable biomaterial for medical application and tissue regeneration has resulted in the extensive research of surface functionalization of material. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial polymer well-known for its high levels of biocompatibility, non-genotoxicity, and minimal tissue response. We have designed a porous antimicrobial silver SSD blend/poly(3HB-co-4HB)-collagen peptide scaffold using a combination of simple techniques to develop a scaffold with an inter-connected microporous pore in this study. The collagen peptide was immobilised via -NH2 group via aminolysis. In order to improve the antimicrobial performance of the scaffold, silver sulfadiazine (SSD) was impregnated in the scaffolds. To confirm the immobilised collagen peptide and SSD, the scaffold was characterized using FTIR. Herein, based on the cell proliferation assay of the L929 fibroblast cells, enhanced bioactivity of the scaffold with improved wettability facilitated increased cell proliferation. The antimicrobial activity of the SSD blend/P(3HB-co-4HB)-collagen peptide in reference to the pathogenic Gram-negative, Gram-positive bacteria and yeast Candida albicans exhibited SSD blend/poly(3HB-co-4HB)-12.5 wt% collagen peptide as significant construct of biocompatible antibacterial biomaterials. Thus, SSD blend/P(3HB-co-4HB)-collagen peptide scaffold from this finding has high potential to be further developed as biomaterial.

Highlights

Biomaterials serve as an interface with the biological systems to evaluate, augment, treat, or replace any tissue, organ or function of the body [1]
Collagen peptide was immobilized onto the porous surface via aminolysis
Salt-leaching is a simple technique where desirable pore sizes were created by flushing the porogen with water, leaving behind pores formed by the porogen [18]

Summary

Introduction

Biomaterials serve as an interface with the biological systems to evaluate, augment, treat, or replace any tissue, organ or function of the body [1]. The success of biomaterials depends on several factors that determine their appropriate interaction with the host biological environment. Their physical and chemical properties, as well as topography and chemistry of the surface, have an important influence on cellular response [2]. Surfaces act as crucial platform in biology and medical fields since most of the biological reactions occur at the surface and interfaces [3]. Polymers 2020, 12, 2979 biomaterials requires detailed understanding on the interaction among the biomaterial surface, host tissue cells and extracellular matrix (ECM) to elicit ideal tissue response (Figure 1) [4].

Methods

Results

Conclusion