Abstract
A comparison of repair synthesis after exposure to ultraviolet light (UV) or the antimicrobial silver sulfadiazine (sublethal dose) was made in <i>Pseudomonas aeruginosa, </i>strain 2 (ATCC 15693), by use of a D,<sup>15</sup>N,<sup>13</sup>C density labeling system. During the initial 15 min of incubation after treatment with both agents, both a ‘repair’ synthesis and a reduced semi-conservative deoxyribonucleic acid (DNA) synthesis occurred. In the sublethal dose range used, the latter was greater than the former. Normal cells showed only a semi-conservative type of replication and, therefore, within the limits of the resolution of the system used (the incorporation of 1,000-5,000 nucleotides per replicating chromosome could be measured), DNA in normal cells did not appear to undergo a repair synthesis involving thymine exchange. The silver sulfadiazine treated cells appear to use a repair mechanism similar to UV irradiated cells. The bacteriostatic-bacteriocidal activity may be related to the rate of drug interaction with <i>Pseudomonas </i>DNA compared to the rate of ‘excision-repair’.
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