Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7

Abstract

The high demand for renewable energy and increased biodiesel production lead to the surplus availability of crude glycerol. Due to the above reason, the bio-based value addition of crude glycerol into various bioproducts is investigated; among them, microbial lipids are attractive. The present study was dedicated to find the optimal glycerol concentration and carbon/nitrogen (C/N) ratio to produce maximum lipid using Yarrowia lipolytica SKY7. The glycerol concentration (34.4 to168.2g/L) and C/N ratio (25 to 150) were selected to investigate to maximize the lipid production. Initial glycerol concentration 112.5g/L, C/N molar ratio of 100, and with 5%v/v inoculum supplementation were found to be optimum for biomass and lipid production. Based on the above optimal parameters, lipid concentration of 43.8%w/w with a biomass concentration of 14.8g/L was achieved. In the case of glycerol concentration, the maximum Yp/s (0.192g/g); Yx/s (0.43g/g) was noted when the initial glycerol concentration was 112.5g/L with C/N molar ratio 100 and inoculum volume 5%v/v. The glycerol uptake was also noted to increase with the increase in glycerol concentration. At low C/N ratio, the glycerol consumption was found to be high (79.43g/L on C/N 25) whereas the glycerol consumption was observed to decrease when the C/N ratio was raised to 150 (40.8g/L).