Abstract
Elongation factor (EF) is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β′ from Spodoptera exigua (SeEF-1β′), its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04 kDa and pI of 4.53. Northern blotting revealed that SeEF-1β′ mRNA is expressed in brain, epidermis, fat body, midgut, Malpighian tubules, ovary and tracheae. RT-PCR revealed that SeEF-1β′ mRNA is expressed at different levels in fat body and whole body during different developmental stages. In RNAi experiments, the survival rate of insects injected with SeEF-1β′ dsRNA was 58.7% at 36 h after injection, which was significantly lower than three control groups. Other elongation factors and transcription factors were also influenced when EF-1β′ was suppressed. The results demonstrate that SeEF-1β′ is a key gene in transcription in S. exigua.
Highlights
Initiation, elongation and termination are the three main steps in translation
SeEF-1β' cDNA has an open reading frame of 672 nucleotides (Figure 1), which encodes a protein of 223 amino acids with a predicted mass of approximately 24.04 kDa and a pI of 4.53
The nucleotide sequence reported in this paper has been submitted to GenBank under accession no
Summary
Elongation factor is a highly conserved protein that plays a role in peptide elongation during translation [1,2,3,4] and is required for protein biosynthesis, with effects such as regulation of protein biosynthesis and acceleration of apoptosis [5]. Translation requires the specific attachment of amino acids to tRNAs by aaRS and subsequent delivery of aa-tRNAs to the ribosome by elongation factor. EF-lA plays an essential role in protein biosynthesis in eukaryotic cells [1]. Recent data for EF-Tu, the analogous elongation factor in prokaryotic cells and eukaryotic organelles, suggest that this protein is partly responsible for proofreading the interaction of codon and anticodon, suggesting that it plays a direct role in the fidelity of translation [15,16]. RNA interference (RNAi) was used to study the function of the gene
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