Abstract
Identifying host factors provides an important clue to understand virus infection. We selected 10 host factor candidate genes and each gene was silenced in Nicotiana benthamiana (N. benthamiana) to investigate their roles in virus infection. The resulting plants were infected with Tobacco mosaic virus (TMV). The accumulation of viral coat protein and the spread of virus were greatly reduced in the plants that eukaryotic translation elongation factor 1A (eEF1A) or 1B (eEF1B) was silenced. These results suggest both eEF1A and eEF1B are required for TMV infection. We also tested for interactions between the eEFs and viral proteins of TMV. Both eEF1A and eEF1B proteins interacted directly with the methyltransferase (MT) domain of the TMV RNA-dependent RNA polymerase (RdRp). eEF1A and eEF1B also interacted with each other in vivo. Our data suggest that eEF1B may be a component of the TMV replication complex which interacts with MT domain of TMV RdRp and eEF1A.
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