Abstract
Liver and adipose tissues are major regulators of lipid metabolism. The aim of this study was to determine the anti‐lipogenic mechanisms of ellagic acid (EA), a naturally occurring polyphenol found in many berries and grapes, in adipocytes and hepatocytes. 3H‐olecic acid and 3H‐acetyl CoA were added to the primary cultures of human adipocytes and human hepatoma Huh7 cells to determine the effect of EA on fatty acid (FA) uptake, triglyceride (TG) esterification and TG de novo synthesis. In adipocytes, EA significantly reduced adipogenic gene expression and TG accumulation. Despite the substantial decrease of TG levels, EA had no significant impact on FA uptake or FA conversion into TG compared to the control group. However, the conversion of acetyl CoA into TG was dramatically reduced. In Huh7 cells, EA decreased TG accumulation in a dose‐dependent manner. Intriguingly, EA in liver cells did not only attenuate de novo synthesis of TG but also decreased FA uptake, and TG esterification. In addition, oxygen consumption was augmented by EA in Huh7 cells. Collectively, these data suggest that EA can attenuate TG contents in adipocytes as well as hepatocytes by altering distinct metabolic pathways.
Published Version
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