Abstract
Plants that synthesize bioactive compounds that have high antioxidant value and elicitation offer a reliable in vitro technique to produce important nutraceutical compounds. The objective of this study is to promote the biosynthesis of these phenolic compounds on a large scale using elicitors in date palm cell suspension culture. Elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2), and silver nitrate (AgNO3) at 50, 100, and 200 mg/L concentrations are used. The effects of elicitors on cell culture were determined in terms of biomass [packed cell volume (PCV), fresh and dry weight], antioxidant activity, and phenolic compounds (catechin, caffeic acid, kaempferol, apigenin) were determined using high-performance liquid chromatography (HPLC). Results revealed that enhanced PCV (12.3%), total phenolic content [317.9 ± 28.7 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)], and radical scavenging activity (86.0 ± 4.5%) were obtained in the 50 mg/L SA treated cell culture of Murashige and Skoog (MS) medium. The accumulation of optimum catechin (26.6 ± 1.3 µg/g DW), caffeic acid (31.4 ± 3.8 µg/g DW), and kaempferol (13.6 ± 1.6 µg/g DW) was found in the 50 mg/L SA-treated culture when compared to the control. These outcomes could be of great importance in the nutraceutical and agronomic industries.
Highlights
The date palm (Phoenix dactylifera L.) is a desert fruit tree known as the “tree of life” for its many uses; it is mainly distributed in Middle Eastern and North African countries [1]
The current study evaluated the effects of various elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2 ), and silver nitrate (AgNO3 ) on the packed cell volume (PCV), fresh weight (FW) and dry weight (DW), total phenol and flavonoid contents, antioxidant activity, and production of catechin, caffeic acid, kaempferol, and apigenin in cell suspension culture of the date palm
Cell suspension cultures of date palm treated with various elicitors such as pectin, SA, CdCl2, and AgNO3 (50, 100, and 150 mg/L concentrations each elicitor) showed decreased PCV, FW, and DW as the concentration increased, except for the YE-treated cell culture, whereas PCV, FW, and DW accumulation decreased as the concentration of the elicitor increased (Figure 1)
Summary
The date palm (Phoenix dactylifera L.) is a desert fruit tree known as the “tree of life” for its many uses; it is mainly distributed in Middle Eastern and North African countries [1]. Date fruits are considered as sacred and are good sources of essential dietary minerals with important medicinal and nutritional values [1,2]. These fruits possess a wide range of phenolic compounds with antioxidant activity [3]. Plants synthesize bioactive compounds naturally as a defense mechanism in response to pathogen attacks. The same nature and response have been found in plants when treated with chemicals of pathogenic origin (elicitors). Elicitors are stress inducers that stimulate secondary pathways resulting in the synthesis of bioactive compounds. Abiotic and biotic elicitors were employed in vitro to induce
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.