Abstract
In the presence of S- adenosyl- l-methionine , enzyme preparations from carrot ( Daucus carota L.) cell suspension cultures specifically catalyze the methylation of caffeoyl-CoA to feruloyl-CoA. Caffeate, 5-O- caffeoyl- d-quinate , 5- O-caffeoylshikimate and methyl caffeate do not act as substrates. The caffeoyl-CoA 3- O-methyltransferase activity is stimulated in vitro by 1 mM Mg 2+. The reaction is inhibited by S- adenosyl- l-homocysteine but not by coenzyme A. Extractable enzyme activity is stimulated by cell wall preparations from Phytophthora megasperma f. sp. glycinea indicating that feruloyl-CoA is an intermediate in the defence reaction of the cells.
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