Abstract
Methods We constructed gp120, gp120-OD, and a trimeric form of gp120-OD (ODx3) based on an M group consensus sequence. Proteins were expressed in 293 cells, and their antigenic properties were evaluated by immunoprecipitation using gp120 bnAbs (b12, 2G12 and 447-52D) and by surface plasmon resonance (SPR). Rabbits were immunized and antibody responses were characterized by ELISA and neutralization assays.
Highlights
Gp120 elicits strong antibody responses, it fails to induce broadly neutralizing antibodies
Proteins were expressed in 293 cells, and their antigenic properties were evaluated by immunoprecipitation using gp120 broadly neutralizing antibodies (bnAbs) (b12, 2G12 and 447-52D) and by surface plasmon resonance (SPR)
Rabbits were immunized and antibody responses were characterized by ELISA and neutralization assays
Summary
Gp120 elicits strong antibody responses, it fails to induce broadly neutralizing antibodies (bnAbs). One strategy being evaluated is using immunogens based on gp120 outer domain (gp120-OD). A number of gp120OD constructs have been reported. None of them have been shown to induce potent nAbs. Here, we describe gp120-OD-based immunogens that can induce potent nAbs
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