Elevated urinary 8-isoprostaglandin F 2α in females with Graves’ hyperthyroidism

Abstract

In this study, we investigate how oxidative stress alters the urinary F 2-isoprostanes’ level and we examine the correlation between 8-isoPGF 2α and thyroid hormones in female patients with Graves’ hyperthyroidism. We quantitatively determined the concentrations of urinary F 2-isoprostanes using gas chromatography-mass spectrometry in the selected ion-monitoring mode. We recruited individuals in the following three groups of subjects for this study: (1) female hyperthyroidism patients ( n=14, 21–71 years), (2) female hypothyroidism patients ( n=16, 16–66 years), and (3) female age-matched normal controls ( n=10, 20–61 years). The average concentration of 8-iso prostaglandin F 2α (8-isoPGF 2α) in hyperthyroidism patients was significantly higher than that in the healthy controls ( P<0.05). The concentration of 8-isoPGF 2α in hypothyroidism patients was similar to that in normal controls. Although the level of 8-isoPGF 2α in two of the patients was slightly elevated, the P value was not significant (NS). Our data suggest that the increased level of urinary 8-isoPGF 2α may reflect possible oxidation injuries in hyperthyroidism patients due to the high level of thyroid hormones. Therefore, the elevated 8-isoPGF 2α in patients could be used as an important biomarker for hyperthyroidism disease.