Abstract
Elemental localization in biological specimens, using x-ray microanalysis, can best be demonstrated with x-ray images. X-ray images provide useful detail about elemental localization as well as relative quantitative information. The comparison of different elements using color overlay images graphically demonstrates interrelationships in ultrathin sections. Pathology specimens with unknown crystal formations were studied using x-ray imaging.Formalin fixed tissue from autopsy and surgical pathology were examined. For this study lung, lymph nodes and skin were used. Some specimens were removed from paraffin blocks and deparaffinized prior to processing for electron microscopy. Tissues were dehydrated and embedded in PolyBed epoxy resin (Polysciences, Warrington, PA). No osmium postfixation was used. Thin unstained sections were coated with carbon using a vacuum evaporator. The sections were examined and analyzed using a Philips EM 400 TEM/STEM microscope equipped with an EDAX PV9900 microanalyzer with the EDAX imaging package. X-ray images were collected using dwell times varying from 10 msec to 1 sec. Longer dwell times resulted in collection times as long as 14 hrs.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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