Abstract
Electrostatic Template-Assisted Deposition (ETAD) of microparticles is described as a new process to control the deposition of microparticles by electrospraying onto a substrate.
Highlights
One of the main goals in cell biology is to nd the optimal in vitro micro-environment to accurately mimic the in vivo one
On the contrary, when the patterned collector was not previously covered by a layer of electrospun nano bers, HA electrosprayed microparticles were deposited both on the pattern walls and in the pattern cavities. This experiment proves without any doubt, that the electrostatic template-assisted deposition (ETAD) of electrosprayed particles is induced by the layer of bers, which
Our goal is to demonstrate that the Electrostatic Template-Assisted Deposition (ETAD) process can be applied for the elaboration of microstructured functional membranes, which are implemented in a biochip to study the in uence of the microstructuration on the behavior of MG63 osteoblast-like cells
Summary
One of the main goals in cell biology is to nd the optimal in vitro micro-environment to accurately mimic the in vivo one. Cell behaviors such as proliferation, migration and differentiation deeply depend on their micro-environment, which is de ned by different parameters such as the topography, chemistry and stiffness of the substrate and the composition and physiochemical conditions (temperature, pH, O2, CO2) of the cell culture medium.[1,2] Among these parameters, the substrate topography is identi ed as one of the most important impacting the cell fate, especially for stem or bone cells. Using this technique, bers can be organized in the form of 2D9–11 or 3D12–15 microstructured scaffolds, with tailored pore sizes to allow cell colonization and in ltration.[16,17] it has been demonstrated that the topography of the electrospun scaffold has got an important in uence on the cell behavior.[18,19,20]
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