Abstract
Ocular chemical injury hit on cornea and conjunctiva due to chemical contact. This injury damages to the epithelial surface of the eye, cornea, anterior segment and permanent unilateral or bilateral permanent viscous damage). Nowadays, human amniotic membrane, allograft biomaterials is used as the treatment for the injury. However, this method has many obstacles. Collagen-based Scaffold is a new study of electrospinning membrane made of collagen, hyaluronic acid, and polyethylene oxide. This research is aimed to obtain the optimum concentration of polymer solution that produces the best morphology in the formation of electrospun collagen-based scaffold. The result show that the fiber had mean diameter of 212, 63 nm to 500, 8 nm and mean pore diameter 498, 56 until 2788 nm. FTIR test shows the loss of functional groups Amide A and Amide II indicate successful crosslinking process with glutaraldehyde. The cytotoxicity test showed electrospun collagen-based scaffold was nontoxic.
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