Electrospinning carbon fibers based molecularly imprinted polymer self-supporting electrochemical sensor for sensitive detection of glycoprotein

Abstract

For glycoprotein detection that is both specific and sensitive in clinical diagnosis and treatment, it is necessary to develop artificial bionic recognition materials. By integrating boronate affinity controllable oriented surface imprinting with carbon nanofibers (CNF) as self-supported electrodes, a simple and cost-effective method for preparing glycoprotein-imprinted self-supported electrochemical biosensors was presented in this paper. The molecular imprinted polymers (MIPs) electrode based on Co, Mo2C-CNF was prepared in the presence of horseradish peroxidase (HRP) as a model glycoprotein by cyclic voltammetry. The CNF mats, composed of Co and Mo2C nanoparticles encapsulated in carbon nanofibers (Co, Mo2C-CNF) via electrospinning, could offer more electrical conductivity and active sites for the detection process. The results demonstrated that the hybrid structure of Co, Mo2C-CNF and MIPs exhibited high specificity and excellent selective recognition efficiency for HRP. The detection limit was obtained as 7.4 fg mL−1. The developed biosensor was applied to detect HRP in human serum with a recovery ranging from 89.21 % to 116.68 %. The developed MIPs electrochemical sensor has great potential for developing highly sensitive and specific glycoprotein imprinting biosensors.