Abstract
Plasmid pKK1 (49.4 Mda), which encodes for Ag+ resistance, was isolated fromPseudomonas stutzeri AG259 (pKK1) and introduced intoPseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1,P. putida CYM318 became resistant to AgNO3. This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation.
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