Electrophysiology of the Neurons in the Area of the Enkephalinergic Magnocellular Dorsal Nucleus of the Guinea‐pig Hypothalamus, Studied by Intracellular and Whole‐cell Recordings

Abstract

The electrophysiological characteristics of 103 hypothalamic neurons in the area of the guinea-pig enkephalinergic magnocellular dorsal nucleus were studied in a thick slice preparation with sharp microelectrodes (63 neurons) and patch pipettes for whole-cell recordings (40 neurons). Of the sampled cells, 79.6% displayed tetrodotoxin-resistant, calcium-dependent slow-depolarizing potentials when the membrane potential was hyperpolarized to approximately -70 mV (type I neurons). Half of them showed robust slow depolarizing potentials, generating bursts of fast action potentials. In the remaining neurons, the slow-depolarizing potentials did not cause burst-firing action potentials but triggered single action potentials. The other class of neurons (20.4% of the sample: type II neurons) did not exhibit calcium-dependent slow-depolarizing potentials. Resting potential, input resistance and the membrane time constant did not distinguish among the two classes of neurons. Current-voltage relationships were heterogeneous. A transient outward rectification was observed in the two classes. This was not totally blocked by 2 mM 4-aminopyridine but was abolished when using perfusion with cobalt instead of calcium. Input resistance and the time constant were higher when measured in the whole-cell mode but the other electrical parameters and the sampling of the recorded neurons were strikingly similar between the two methods of recording. Intracellular staining of 22 neurons retrogradely labelled from the lateral septum allowed confirmation of their location within the magnocellular dorsal nucleus. The study indicates that the electrical properties of these neurons did not differ from those of neurons found throughout the area explored. It also indicates the presence of distinct electrophysiological types of cells in the magnocellular dorsal nucleus, although the nucleus is composed of a single type of enkephalinergic neuron. It provides a basis for the study of the regulation of activity of the neurons at the origin of an enkephalinergic tractus which is involved in neuroendocrine, psychoneuroendocrine and immune processes.