Abstract
1. 1. Hemoglobin and plasma proteins of two species of toads ( Bufo fowleri and Bufo valliceps) and their natural, F1 hybrid were studied by paper, moving- boundary and starch-gel electrophoresis. 2. 2. Each species possessed a single hemoglobin and a single transferrin. Both hemoglobins and transferrins of the two species migrated at strikingly different rates. 3. 3. The hybrids possessed two hemoglobins and two transferrins, one of each protein from each parent species. The electrophoretic mobilities of the hemoglobins were modified when mixed either artificially or naturally (hybrid hemolysates). 4. 4. The two species possessed several plasma protein fractions in common but a few were specific to each species. Considerable individual variation in concentration of certain plasma fractions was noted. 5. 5. All electrophoretic fractions present in the parent species occured in the hybrid. With one possible exception, no fraction was found in the hybrid that was not present in one or both of the parent species. 6. 6. Hybrid plasma contained a higher concentration of protein than the plasma of either parental species.
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