Abstract
A method is described for separating and quantitating the isozymes of creatine kinase in various tissue extracts. We have tested the sensitivity and precision of the method over a wide range of mixtures of isozymes. The quantitation is linear in the range of 0–5 mIU of CK analyzed, and the lower limit of quantitation of an isozyme in a mixture is 10 mIU/ml. For certain tissues the presence of dithiothreitol is necessary to prevent inactivation of the brain-type isozyme.
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