Enzyme hybridization, a method allowing the detection of inactive enzymes in tissue extracts: Application to creatine kinase

Abstract

Hybridization was performed between two isozymes, one active and one inactive, of rabbit creatine kinase. Isozyme BB (fast, brain type), completely inactivated with iodoacetamide, can be hybridized in 8 M urea with isozyme MM (slow, muscle type). Electrophoretic separation followed by specific staining shows the formation of an active hybrid, without reactivation of the alkylated brain isozyme. This hybridization technique seems to be the first nonimmunological method capable of detecting an inactive enzyme in solution.