Abstract
Horizontal starch gel electrophoresis was used to distinguish the larvae of the northern corn rootworm, Diabrotica barberi Smith and Lawrence, and the western corn rootworm, D. virgifera virgifera LeConte. In a survey of 20 enzyme systems, consistent electrophoretic differences were found in four: isocitrate dehydrogenase (IDH), esterase (EST), acid phosphatase (ACPH), and hexokinase (HK). Second and third instars of the two species were accurately identified with IDH and EST, while ACPH and HK were suitable back-up systems. The differences in these isozymes were consistent in two populations of separate geographic origin (South Dakota and Ontario). The use of electrophoresis proved to be a valuable technique for the precise identification of the NCR and WCR larvae of these species, even when the specimens were damaged during extraction from the soil.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
