Electrophoretic deposition of hyaluronic acid-g-dopamine/bovine hemoglobin micelles for functional coatings

Abstract

A novel functional coating was prepared from self-assembled micelles through direct electrophoretic deposition. The micelles were obtained via self-assembly of amphiphilic hyaluronic acid-g-dopamine (HA-g-DA) in aqueous solution with encapsulation of bovine hemoglobin (BHb). The size, morphology, and zeta potential of the resulting micelles were characterized. Then, the micelles were immobilized onto conductive substrate using electrophoretic deposition technique to fabricate nanostructured coating. The structure and morphology of the resulting coating were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The release behavior of BHb from the coating was evaluated in a simulated body fluid (SBF), and the BHb was released up to 20 days. And in vitro assay demonstrated that the nanostructured coating is biocompatible to NIH3T3 and Hela cells, respectively. The contribution of our work lies in the creation of a novel strategy to fabricate a functional coating in viewing of the versatility of self-assembled micelles and the controllability of electrophoretic deposition process. It is believed that our work provides new ideas and reliable data to design functional coatings for biomedical applications.