Abstract
A total of 210 samples of haemoglobin of Trachurus trachurus (L.) caught in the Gulf of Trieste in 1972–1973, were analysed in search of haemoglobin polymorphism. Genetic polymorphism may be used as a genetic marker to identify sub–populations on the basis of allele frequencies.All the samples analysed produced the same haemoglobinic pattern, composed of six bands. The quantitative differences noted between the various fractions were not sufficient to demonstrate the existence of different phenotypes.Denaturation tests carried out by treating erythrocytes with non–sterile physiological solutions or by keeping the haemolysate at room temperature before electrophoresis, resulted in new patterns. This result shows the importance of the careful treatment of blood samples to assure reliable results.
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