Electrophoresis Study of Wheat (&amp;lt;i&amp;gt;Triticumaestivum&amp;lt;/i&amp;gt; L.) Protein Changes Under Salinity Stress

Neda Sobhanian

doi:10.11648/j.sr.20160402.12

Abstract

Salinity problem with its vast spread on the earth is one of the main factors which limits crop production. One of the methods to overcome this problem is taking advantage of the resistant genotypes. Investigation of changes resulting from the stress in an electrophoretic profile of proteins and understanding its relation with the tolerance and susceptibility of cultivars is an important criterion for identifying such cultivars. In this study, 4 wheat cultivars with different degrees of tolerance were grown in hydroponic culture under salinity treatment (0, 70, 140 and 210 mM NaCl). Leaf sampling was done on 5 leaf stage. Studying the electrophoretic pattern of the leaf soluble proteins in salinity and control treatments showed fundamental similarities among the cultivars. No polypeptide bands belonging to the specific cultivars or to one of the salinity treatments were observed. The study of protein changes by electrophoretic analysis under salinity treatment may be useful for understanding the salinity tolerance of genotypes.

Highlights

Salinity is one of the most destructive abiotic stresses that constraint crop production and growth in many parts of the world [1]
Neda Sobhanian et al.: Electrophoresis Study of Wheat (Triticumaestivum L.) Protein Changes Under Salinity Stress
No polypeptides belonging to the specific cultivars or to one of the salinity treatments were observed

Summary

Introduction

Salinity is one of the most destructive abiotic stresses that constraint crop production and growth in many parts of the world [1]. Salinity has adverse effects to most crops. The negative effects of salinity are due to increase in Na+ and Cl– ions in different plants. Salinity at higher levels has different adverse effects. It causes membrane damage, nutrient imbalance, altered levels of growth regulators, enzymatic inhibition and metabolic malfunction, including photosynthesis which leads to plant death [9]. One way to resolve these problems is improvement of crops that are more tolerant to salinity stress. One of the most important character for salt tolerance is low level of Na+ and Cl– ions absorb and transition to the leaves [12]

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