Protein pattern analysis in tolerant and susceptible wheat cultivars under salinity stress conditions

Marouf Khalili,Mohammad Reza Naghavi,Said Yousefzadeh

doi:10.14720/aas.2018.111.3.03

Abstract

<p>To investigate proteome pattern of wheat cultivars, young leaves were collected from tillering stage of seedlings two weeks after development of the salinity stress. The extraction of proteins from leaf tissue was done and two dimensional electrophoresis using IPG strips and SDS-PAGE in the control and salinity treatments were performed. In total, 198 and 203 protein spots were identified in tolerant (‘Moghan3’) and susceptible (‘Pishtaz’) cultivars respectively. Also, among these, spots number 21 and 22 were detected with significant IF in ‘Moghan3’ and ‘Pishtaz’ respectively. Two-stage mass spectrometry (MS/MS) was used to identify protein spots. Common identified proteins, including proteins involved in removal of oxidants, Calvin cycle proteins, proteins involved in light reaction of photosynthesis and proton transfer, and heat shock protein were identified on basis of the functional groups and their frequency. In total, ‘Moghan3’ maintained the stability of the structure and performance of carbon metabolism under stress better than susceptible cultivar. In addition, defense against oxidative stress induced by salinity stress was performed by 2-cys peroxiredoxin BAS1 and Cu-Zn SOD proteins that tolerant cultivar defended against oxidative stress better than the susceptible cultivar. The greatest strength of ‘Moghan3’ and major weakness in ‘Pishtaz’ are relying on the unique proteins formed under salinity stress for the removal of oxidants and to maintain the activity of the photosynthetic light reactions, respectively.</p>

Highlights

Salinity stress tolerance in plants is a complex phenomenon and it is communicated with the physiological, biochemical and molecular mechanism
Proteomic approaches are identified as one of the most important methods for understanding the molecular basis of salt stress tolerance at the protein levels (Thiellement et al, 2002). Abiotic stresses such as salinity, before any effects are detected at the production level cause the change in cytoplasmic calcium concentration and pH which is understood as the main plant response mechanism under these condition
Due to reduced expression or lack of expression of this protein in the ‘Pishtaz’, the activity of photosystem II and eventually the efficiency of photosynthetic light reaction be reduced in this cultivar while this protein (Spot No 15) showed increased expression in tolerant cultivar (‘Moghan3’). These results indicate that under salinity stress, one of the important components of the photosynthesis machine, namely the oxygen swirling and the photosystem II complex has strongly affected, contributing to the aging of the leaves and possibly the gradual death of the cells

Summary

Introduction

Salinity stress tolerance in plants is a complex phenomenon and it is communicated with the physiological, biochemical and molecular mechanism In this regard, proteomic approaches are identified as one of the most important methods for understanding the molecular basis of salt stress tolerance at the protein levels (Thiellement et al, 2002). Proteomic approaches are identified as one of the most important methods for understanding the molecular basis of salt stress tolerance at the protein levels (Thiellement et al, 2002) Abiotic stresses such as salinity, before any effects are detected at the production level cause the change in cytoplasmic calcium concentration and pH which is understood as the main plant response mechanism under these condition. Salinity and drought stresses overlap on physiological level because salt in soil decreases the amount of available water and leads to reduced water absorption (Tuteja, 2007)

Methods

Results

Conclusion