Abstract
Respiratory chain composition and energy coupling in cytoplasmic membrane of Zymomonas mobilis was shown to depend on culture aeration. Aerobically grown cells contained mainly the non-energy-generating NADH dehydrogenase with kM for NADH 58 mM. In anaerobically cultivated bacteria, the energy-coupling NADH dehydrogenase complex with km for NADH 7 mM predominated. In aerobically cultivated Z. mobilis, CoQ content and absorption peaks of cytochromes at 554–556 nm and 525–528 nm were significantly increased. Energy-coupling site I, linked to the NADH:CoQ oxidoreductase complex, could be eliminated under sulfate-déficient cultivation conditions. For anaerobically grown cells this resulted in loss of oxidative phosphorylation activity, while in aerobically grown cells energy coupling was not affected. These findings indicate a shift of energy coupling from site I to the cytochrome region of the respiratory chain in Z. mobilis under transition from anaerobic to aerobic growth conditions.
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