Electron beam irradiation synthesis of porous and non-porous pectin based hydrogels for a tetracycline drug delivery system

Abstract

Electron beam irradiation was used for the synthesize of porous and non-porous pectin based hydrogels through conjugation of pectin with 5-hydroxytryptophan in the presence and absence of sodium dodecyl sulfate, respectively. The hydrogels were characterized by different methods. Tetracycline hydrochloride was loaded on the hydrogels by swelling equilibrium method and its release in different media was studied. The effect of SDS amount and electron beam irradiation dose on the swelling, drug loading, drug release, gel content, and mechanical properties were investigated. The release of drug form both hydrogels followed non-Fickian diffusion mechanism and was best fitted with the Korsmeyer-Peppas model. In vitro, drug release studies revealed that the release of drug from non-porous hydrogel is relatively slow while its release from porous hydrogel occurs with higher amounts and faster rate. Biocompatibility and cytotoxic analysis indicate that the prepared hydrogels dressing are non-thrombogenic, non-hemolytic and non-toxic. Furthermore, acceptable bacteria growth inhibition against Escherichia coli and Staphylococcus aureus were observed for both drug-loaded hydrogels. Thus, these hydrogels are suitable for the application of an antibacterial wound dressing.