Abstract
The electrochemical reduction and oxidation of hemoglobin at a Brilliant Cresyl Blue (BCB) covered electrodes prepared by simple adsorption as well as by the cyclic potential scanning technique have been measured using UV—Vis spectroelectrochemistry. These electrodes exhibit the ability to catalyze the redox processes of hemoglobin with higher efficacy. The formal heterogeneous electron transfer rate constant is k 5 = 3.7 × 10 −7 cm/s. The adsorption of BCB at platinum electrodes was confirmed by ESCA. The stable free radical and a possible triplet state of BCB formed during the electrode processes mediate the reduction and oxidation of hemoglobin.
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