Electrode processes of hemoglobin at a platinum electrode covered by Brilliant Cresyl Blue

Abstract

The electrochemical reduction and oxidation of hemoglobin at a Brilliant Cresyl Blue (BCB) covered electrodes prepared by simple adsorption as well as by the cyclic potential scanning technique have been measured using UV-Vis spectroelectrochemistry. These electrodes exhibit the ability to catalyze the redox processes of hemoglobin with higher efficacy. The formal heterogeneous electron transfer rate constant is k s = 3.7 × 10 −7 cm/s. The adsorption of BCB at platinum electrodes was confirmed by ESCA. The stable free radical and a possible triplet state of BCB formed during the electrode processes mediate the reduction and oxidation of hemoglobin.