Abstract
In this work, the interaction between theophylline (TP) which gives a stable well-defined reduction peak at (1.07) V versus Ag/AgCl. Sat. KCl/ in phosphate buffer solution (pH=7) and urea was studied using square wave voltammetry (SWV) technique. Also, the binding constant and the thermodynamics parameters have been calculated. Different temperatures (288, 293, 298, 298, 303, 308 and 310) ºK were used to study the effect of temperature on binding constant (K). The results showed that the binding constant (K) decreased with increasing temperature. This is as a result of the negative value of enthalpy (-31.07)KJ.mol-1. The negative value of Gibbs energy (-13.632 x 102_ -12.296 x 102) KJ.mol-1 indicates that the interaction is spontaneous and could be due to van der Waals forces or hydrogen bonds effect (weak interaction).).
Highlights
EXPERIMENT ApparatusAll the electrochemical experiments were performed using a797VA Computrace instrument (Metrohm, Switzerland)
In this work, the interaction between theophylline (TP) which gives a stable well-defined reduction peak at (1.07) V versus Ag/AgCl
Many methods have been employed for measuring theophylline quantitatively, such as liquid chromatography (Kalyani et al, 2017; Srdjenovic et al, 2008), UV spectrometry (Sujana et al, 2016; Culzoni et al, 2005), chemiluminescent immunoassay (Zhou et al, 2005), gas chromatographymass spectrometry (GC–MS)and gas chromatography–isotope dilution mass spectrometry (GC– IDMS) ( Arinobu et al, 2009; Kress et al, 2002)
Summary
All the electrochemical experiments were performed using a797VA Computrace instrument (Metrohm, Switzerland). The reference electrode was an Ag/AgCl with saturated KCl and a platinum wire was used as the auxiliary electrode and a glassy carbon electrode GC used as the working electrode. PH measurements were performed by using a digital pH meter The Haake Heated Water Bath Circulator is Model G, USA. Chemicals and Reagents All chemicals used in this work K2HPO4, and potassumdihydrogen phosphate KH2PO4) were of analytical grade and used without further purification, and were purchased from Fluka, and BDH
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