Abstract
AbstractThe redox mediator Meldola blue (MB) was entrapped into two polymers, poly‐1,2‐diaminobenzene (p‐DAB) and poly‐3,4‐ethylenedioxythiophene (p‐EDOT) by potential cycling and films were applied to NADH oxidation with subsequent glutamate detection using immobilized glutamate dehydrogenase. Both polymer films were tested for electrocatalysis of NADH using amperometry at Eapp=0.1 V vs. Ag/AgCl and similar response characteristics were obtained with sensitivity values of 6.1 nA μM−1, linear range up to 0.5 mM (R2=0.9972) and LOD of 50 μM. Subsequent amperometric determination of glutamate resulted in sensitivity 0.7 nA μM−1, linearity 0–100 μM and detection limit of 2 μM glutamate.
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