Electrochemical, Real-Time Monitoring of Hydrogen Peroxide in Drosophila (Fruit Fly) Intestines Using a Carbon Fiber Microelectrode (CFME) Modified with Platinum Nanoparticles (PtNPs)

Abstract

Inflammatory bowel disease (IBD) is a serious global health problem, mainly manifested as chronic progressive gastrointestinal inflammation, which damages the gastrointestinal mucosa and affects the life of human beings. This disease is difficult to eradicate, prone to recurrent attacks, has a potential cancer risk and includes Crohn’s disease (CD) and ulcerative colitis (UC). Unfortunately, the available drugs serve to induce remission but may result in adverse reactions and drug dependence. However, in the case of inflammation-related diseases, it is possible to assess and measure the severity of the disease and the impact of treatment by monitoring the levels of hydrogen peroxide (H2O2). Moreover, in-situ detection is of great significance as it represents the inflammatory site. In this study, a carbon fiber microelectrode modified by platinum nanoparticles was prepared and used to monitor H2O2 in intestine of an UC Drosophila model established by dextran sodium sulfate. The results show that the electrochemical biosensor provides sensitive, real-time determination of H2O2 in vivo, which provides a new methodological basis for studying the occurrence and development of UC and drug screening.