Electrochemical Investigation of Curcumin-DNA Interaction by Using Hydroxyapatite Nanoparticles-Ionic Liquids Based Composite Electrodes.

Merve Uca,Ece Eksin,Arzum Erdem,Yasemin Erac

doi:10.3390/ma14154344

Abstract

Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis.

Highlights

In recent years, electrochemical nucleic acid biosensors have gained interest since they enable the observation of specific DNA hybridization and DNA interactions
Hydroxyapatite nanoparticles (HaP)- and ionic liquid (IL) modified electrochemical DNA biosensor was introduced for the first time in the present study
Curcumin was chosen as the DNA-targeted molecule since it is known that the interaction of curcuminoids and their metal complexes with DNA occurs through groove binding or intercalation

Summary

Introduction

Electrochemical nucleic acid biosensors have gained interest since they enable the observation of specific DNA hybridization and DNA interactions. These types of biosensors supply high sensitivity, simplicity, cost-effectiveness, and suitability for microfabrication [1,2,3]. Hydroxyapatite (HaP), is one of the apatite mineral family [6] and is similar to the mineral constituent of bone. It is biocompatible, bioactive, and has particular multi-adsorbing sites [7]. Erdem and Congur [7] developed hydroxyapatitenanoparticles-modified pencil graphite electrode for the first time, and sequence-selective DNA hybridization was monitored by DPV technique

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