Abstract
A novel electrochemical immunosensor prepared by direct coating of small molecular hapten on glassy carbon electrode (GCE) is reported for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D). The glassy carbon electrodes were activated by electrochemical method and then were further treated with 3-aminoprpyltriethoxysilane (APS) to functionalize the GCE surface with amino groups for covalent linkage to small molecular hapten of 2,4-D with carboxyl groups. The electrodes were characterized by X-ray photoelectron spectroscopy (XPS). The principle of this immunoassay is based on an indirect competitive immunoassay. Analytes of 2,4-D and hapten of 2,4-D immobilized on glassy carbon electrode (GCE) compete for 2,4-D antibodies labeled by horseradish peroxidase (HRP-anti-2,4-D). After complete immunoreaction, the GCE attached HRP-anti-2,4-D tracers was transferred to a substrate solution containing hydroquinone (QH2) and hydrogen peroxide for square wave voltammetry (SWV) detection. Determination conditions were optimized. The SWV anodic peak current decreased linearly with the increase of 2,4-D concentration over the range from 0.10 to 15.0 mg/L 2,4-D with a detection limit of 0.01mg/L. The performance of this electrochemical immunoassay was successfully evaluated with river water spiked with 2,4-D, indicating that this convenient, rapid, and sensitive technique offers great prospect for the monitoring of trace 2,4-D in environment.
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