Abstract
In order to solve the problems of unstable signal and fastdecrease of response signal of traditional electroactive substances, we synthesized a new type of electroactive substances, so that they can cooperatively amplify the response signal of the immune sensor and further improve its sensitivity. Theconductive organic ligand tetrahydroxy 1,4-benzoquinone (THQ) and Cu<sup>2+</sup> were chelated to form a stable polymeric two-dimensional metal-organic framework material Cu-THQ.Cu-THQ is a new type of electroactive substance. Compared with the traditional electroactive substance, Cu-THQ not only has stronger Redox activity, but also better stability and biocompatibility.We carried out various electrochemical characterizations on Cu-THQ and further proved that the introduction of Cu<sup>2+</sup> not only greatly enhanced the stability of THQ electrical signal, but also increased its signal response.The experimental results showed that theprepared immunosensor exhibited good linearity for the detection of carcinoembryonic antigen in the range 1fg/mL to 40ng/mL, with detection limits and sensitivity of 0.477fg/mL and 14.81 µA/(ng/mL)/cm<sup>2</sup>, respectively.
Published Version
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