Abstract
In the present study, a sensitive and selective impedimetric detection of solution-phase nucleic acid hybridization related to Factor V Leiden (FV Leiden) mutation was performed by carbon nanofibers (CNF) modified screen printed electrodes (SPE). The microscopic and electrochemical characterization of CNF-SPEs was explored in comparison to the unmodified electrodes. Since the FV Leiden mutation is a widespread inherited risk factor predisposing to venous thromboembolism, this study herein aimed to perform the impedimetric detection of FV Leiden mutation by a zip nucleic acid (ZNA) probe-based assay in combination with CNF-SPEs. The selectivity of the assay was then examined against the mutation-free DNA sequences as well as the synthetic PCR samples.
Highlights
Nucleic acid-based technologies have been greatly used in research and diagnostics
The results revealed that the carbon nanofibers (CNF)-screen printed electrodes (SPE)’s exhibited a satisfactory reproducibility of different concentrations of the target DNA, and the change of Rct value was recorded with a mean change of the as 62.55 Ohm and a relative deviation
The results revealed that the CNF-SPE’s exhibited a satisfactory reproducibility with a mean change of the response as 62.55 Ohm and a relative standard deviation of 4.89 %
Summary
Nucleic acid-based technologies have been greatly used in research and diagnostics. In order to get the specific, accurate and sensitive analysis of nucleic acid hybridization, the specific sequence recognition is required to be improved. The efficiency of nucleic acid hybridization can be improved by decreasing the electrostatic repulsion between negatively charged nucleic acid strands. In this aspect, the coupling of oligonucleotides with spermine residues as the cationic units provider has been recently introduced in the literature [1,2,3]. Zip nucleic acids (ZNAs) contain a cationic compound, spermine.
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