Abstract
In this work, a novel strategy to fabricate a highly sensitive and selective biosensor for the detection of Ag+ is proposed. Two DNA probes are designed and modified on a gold electrode surface by gold-sulfur chemistry and hybridization. In the presence of Ag+, cytosine-Ag+-cytosine composite forms and facilitates the ligation event on the electrode surface, which can block the release of electrochemical signals labeled on one of the two DNA probes during denaturation process. Ag+ can be sensitively detected with the detection limit of 0.1 nM, which is much lower than the toxicity level defined by U.S. Environmental Protection Agency. This biosensor can easily distinguish Ag+ from other interfering ions and the performances in real water samples are also satisfactory. Moreover, the two DNA probes are designed to contain the recognition sequences of a nicking endonuclease, and the ligated DNA can thus be cleaved at the original site. Therefore, the electrode can be regenerated, which allows the biosensor to be reused for additional tests.
Highlights
In this work, a novel strategy to fabricate a highly sensitive and selective biosensor for the detection of Ag1 is proposed
We have proposed a novel electrochemical strategy for Ag1 assay based on Ag1-assisted DNA ligation, denaturation and annealing events of two DNA probes
Two oligonucleotides named as capture probe (CP) and signal probe (SP) are employed
Summary
Peng Miao[1,2], Kun Han[1,2], Bidou Wang[1], Gangyin Luo[1], Peng Wang[1], Mingli Chen1 & Yuguo Tang[1,2]. Ag1 can be sensitively detected with the detection limit of 0.1 nM, which is much lower than the toxicity level defined by U.S Environmental Protection Agency. This biosensor can distinguish Ag1 from other interfering ions and the performances in real water samples are satisfactory. A diversity of techniques have been applied for the analytical purposes, such as voltammetry, chronocoulometry, microscope, fluorescent and colorimetric methods[13,14,15,16,17,18,19] In this contribution, we have proposed a novel electrochemical strategy for Ag1 assay based on Ag1-assisted DNA ligation, denaturation and annealing events of two DNA probes. The developed biosensor has great potential use for monitoring Ag1 in real samples
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