Abstract
This work presents the development of a sustainable and biocompatible electrochemical biosensor for the detection of phenolic compounds, leveraging peroxidase from <i>Cucumis anguria</i> (gherkin) homogenate and chitosan‐coated iron oxide nanoparticles (γ‐Fe <sub>2</sub>O <sub>3</sub>@CTS). A simple and cost‐effective method was used to prepare the enzymatic extract, with peroxidase activity confirmed spectrometrically. The γ‐Fe <sub>2</sub>O <sub>3</sub>@CTS were synthesized with hydrodynamic radius of 146.8 ± 8.4 nm. Square wave voltammetry (SWV) was employed to optimize experimental parameters for phenolic detection. Analytical curves were constructed for quercetin, morin, ferulic acid, and 2,5‐dihydroxybenzoic acid (2,5‐DHBA), showing excellent linearity and low detection limits (LOD)
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