Electrochemical and Spectroscopic Studies of Riboflavin

Abstract

Flavin derivatives are highly versatile electroactive molecules, which catalyse a large number of redox reactions in biological systems. In this context, we hereby report the electrochemical studies of riboflavin (Rf) in buffered aqueous solution at varying pH (3.0, 5.0 7.0, 9.0 and 12.0) using cyclic voltammetry (CV) technique. The CV results infer reversible as well as quasi-reversible behaviour of Rf involving two anodic and cathodic peaks. The electrochemical kinetic parameters showed the change in structural behaviour of Rf at different pH conditions and provide insight into the one or two electron transfer reaction. The UV-Vis spectroscopic and photoluminescence (PL) studies of Rf further confirmed its structural changes. This study will help to understand the pH dependence of the biological and non-biological reaction mechanisms carried out by flavoenzymes.